The Proteomics core provides services and support to assist investigators within WCM-Q and other institutions in Qatar or abroad, in addressing and solving the peptide and protein-based challenges inherent to their research fields. Besides pure analytical capabilities, the core also offers additional services, including technological consultation, experimental design optimization, custom sample preparation/analysis method development, as well as high end custom data analytical solutions enterprise software- GeneData.
Facilities
The proteomics core facility is equipped with high resolution quadrupole Orbitrap mass spectrometers (Q-Exactive series, Thermo Scientific) coupled to nano-scale ultra-performance liquid chromatography systems suitable for both exploratory and targeted investigations. Additionally, two nano- to micro-scale high performance liquid chromatography systems equipped with multi wavelength UV detectors and fraction collectors are employed for various sample preparation work-flows: multidimensional separation, post translational modification enrichment, etc...
Alongside the mass spectrometry-based resources, the proteomics core is the first research core laboratory in the Middle East to obtain certification from Olink Proteomics as a service provider apart from the twelve other centers worldwide (link to WCM-Q website news). The Olink™ assay, uses the Proximity Extension Assay (PEA) technology to detect protein biomarkers designed in 14 human proteomics panels across a wide range of concentrations in small volumes (1 uL) of various biological sample types including plasma and serum. This assay is run in 96-well format which includes internal and external control samples.
Services
Services and Resources provided by Proteomics Core:
- Consultation
- Sample preparation
- Exploratory proteomics analysis
- Post translational modification analysis
- High throughput targeted proteomics analysis (affinity-based approach)
- Validation targeted proteomics (mass spectrometry-based approach)
- Quantitative proteomics
- Data analysis
Contact
Frank Schmidt, Ph.D.
Assistant Professor of Biochemistry
Director, Proteomics Core
Weill Cornell Medicine - Qatar
Tel.: +974 4492 8279
Email: frs4001@qatar-med.cornell.edu
Neha Goswami, MSc
Laboratory Supervisor, Proteomics Core
Weill Cornell Medicine - Qatar
Office: +974 4492 8917
Mobile: +974 3363 7848
E-mail: neg2007@qatar-med.cornell.edu