The microscopy core, along with the flow cytometry and histology cores, provides equipment and technical support to help investigators successfully complete research projects. Several state-of-the-art microscopes enable researchers to apply sophisticated optical microscopy techniques to explore cellular structure and specific labeled protein localization. Various plate readers have the capacity to perform high throughput screening.
Carl Zeiss LSM-880 inverted confocal microscope
Equipped with the UV, VIS and IR lasers in combination with 34-channel QUASAR detector design, LSM-880 brings complete freedom to your fluorescence microscopy. LSM-880 enables confocal microscopy for a wide variety of applications, including z-stack, time series, FRET, FRAP and colocalization. The stage mounted CO2 incubator allows prolonged observation of living cells.
Leica SP5 STED confocal microscope
Leica STED is a super-resolution confocal microscope. Stimulated Emission Depletion (STED) is a special illumination technique allowing the resolution of cellular details below 80nm. Multiple laserlines (458, 488, 514, 561, and 633nm) are present for traditional confocal images. Along with a classical line scanner, a resonance scanner is equipped for faster imaging. It allows z-stack, time series, tile scanning, bleaching, etc.
Zeiss SteREO Lumar.V12
The stereo microscope can obtain high-resolution three-dimensional images in the largest specimen field in its class. First-class optics through the new objective NeoLumar S captures bright fluorescence, rich in contrast. Alongside transmitted and reflected light, the filter wheel has fluorescence filters for DAPI, FITC and Cy3. Complete motorization and an innovative operating system enable easy focus and zoom.
Zeiss Epifluorescence Microscope with Colibri
The combination of Colibri (an LED excitation light source) and the AxioVison imaging system results in extremely fast switching times, with precise control of the illumination intensity to protect the sample. For applications requiring higher illumination intensity, Carl Zeiss offers an externally coupled Metal Halide (HXP) white light source. The system is excellent for live cell imaging, high-speed or multi-channel fluorescence microscopy.
ImageXpress Micro XLS Widefield High-Content Screening System (Molecular Device)
ImageXpress Micro XLS System is a widefield automated microscope capable of fluorescent, transmitted light, and phase-contrast imaging of fixed- or live-cell assays, tissues and small organisms. Speed, flexibility, and high quality data are assured with a large field-of-view, industry-leading stage and autofocus control, the broad range of research-grade objective lenses available, and multiple filter options. Combined with MetaXpress Software for acquisition and analysis, the system will help you interpret your images, understand your data, and perform high throughput screening.
FlexStation 3 Multi-Mode Microplate Reader (Molecular Device)
With advanced dual optical systems operating above and below your microplates, the FlexStation® 3 Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Using 8-channel pipettor, the FlexStation 3 reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 distinct wells in a source plate to a read plate, simultaneously. The FlexStation 3 Microplate Reader is supplied with SoftMax® Pro Microplate Data Acquisition & Analysis Software.
Imaris Image Analysis: State-of-the-art image analysis software suite with functionality for the visualization, segmentation, and interpretation of 3D and 4D microscopy datasets.
Khaled Machaca, PhD
Professor of Physiology and Biophysics
Associate Dean for Research
Sun, Lu, PhD
Manager of the Microscopy Core
Office Phone: 4492-8327
Liberska, Aleksandra, MS
Flow Cytometry Supervisor
Office Phone: 4492-8474