PhD, Universit ät Hohenheim
Phone: (974) 4492 8334
Main Research Interests of our laboratory are
1. Chemotherapy and Intracellular Calcium ([Ca2+]i)
2. Modulation of membrane receptors and channels and intracellular pathways by metals and metal compounds.
3. Cellular Mechanisms of Pain Modulation
4. Principles of the Respiratory Rhythm Generation
In our lab we investigate mechanisms regulating clinical as well as toxicological relevant substances (metal ions (e.g. Pb2+, Zn2+ or Al3+), metal compounds (e.g. trimethyltin, cisplatin or arsenic), or biological active substances (e.g. capsaicin, melatonin or TNF alpha) have specific actions on (neuronal) cells: they interfere with their physiological (e.g. modulation of pain reception) or pathophysiological (apoptosis) functions.
These substances activate/deactivate proteins at the cellular membrane, the organelles or in the cytosol that are part of specific intracellular signalling pathways. Therefore our laboratory is interested in the modulation of neuronal communication as well as in their interference with calcium dependent pathways, which could result in the activation/inhibition of apoptotic cascades.
Therefore, our research focuses on different levels of the interaction of drugs or environment/clinical relevant substances with different models. Our working group is interested in the interference of the above mentioned active substances with:
- membrane proteins such as channels and ATPases, and how they modulate membrane currents and the membrane potential (e.g. resulting in a modified excitability of the neurones) (electrophysiological approach);
- intracellular calcium homeostasis particularly calcium entry or calcium release from the stores, which itself will influence the in-/efflux though the calcium channels as well as it will interfere with multiple signalling pathways (imaging and molecular approach) (see attached movie);
- the "cross modulation" of activated/deactivated membrane currents and the intracellular calcium level, to analyse in which way this will result in a modified transmitter release and/or the activation of (calcium dependent) intracellular pathways that could possibly results in an activation of apoptotic pathways (mainly molecular approach) (see figures 1, 2 and 3).
To receive meaningful results which will help to understand cellular mechanisms not only adequate techniques are needed such as electrophysiological, imaging techniques as well as molecular approaches which are all already part of our daily routine but also the combined use of these techniques has to be established e.g. parallel calcium imaging with electrophysiological recordings.
Furthermore, having discovered and understood mechanisms at the cellular side, does not necessarily answer all the questions. Whether the same actions will also occur in an intact tissue or body has to be analyzed also. While testing of some neuronal functions e.g. the long term potentiation (LTP) in brain slices is today well established we also want to prove the action of our substances in a more intact in vitro model ("working heard-brainstem" preparation). In this model we already have demonstrated that different substances influence the intact neuronal network (here the respiratory network of the preBötzinger complex located within the brain stem).
The results of the different approaches will hopefully give new answers, which will help to understand more of the cellular functions. This will give insights, how toxicological and/or clinical relevant compounds interact with the cell and the organism.
Overall I would like to answer the question under which circumstances a specific drug or chemical is (neuro-)toxic and which differences at the cellular side could make the same substance a powerful tool for clinical use (e.g. as an anti-cancer drug).
Post Doctoral fellow position will open by end of 2014
Senior Research Specialist
"Working in Dr. Büsselberg`s Lab is a very exciting and challenging experience. It has provided me the opportunity to acquire skills in lab management and also introduced me to novel techniques in research. Moreover, I have been exposed to a wide variety of tasks including training students & volunteers, reviewing literature, presenting posters, developing research plans, editing and proof reading the scientific papers etc. An exciting part of my experience here, was to be part of the animated review on” Lead (Pb2+) neurotoxicity: Ion-mimicry with calcium (Ca2+) impairs synaptic transmission” published in the Journal of Local and Global Health Science. I enjoyed very much to train the students who joined the lab for their summer research. "
Dr. Khalid Menshawy
"I graduated from Alexandria Faculty of Medicine, Egypt in 2012. I am interested in Neuroscience and Electro-physiology. I am working on neuroblastoma cell receptors using the patch clamp device."
"I am currently a first year medical student in WCM-Q. In addition I have worked in research labs in both Qatar and Ithaca, NY (In the SSRP program) and have gained valuable lab experience in both lab techniques and analyzing data. Recently I took part in a project trying to identify if the use of pain modulating drugs such as aspirin interfere with the effect of anti cancer drugs."
"Our project focuses on examining the effect of pain modulators that affect intracellular calcium on cancer drug potency. Our aim is to relate the resulting cytotoxicity in cancer cells to fluctuations in intracelluar changes in calcium which can be done using calcium imaging with calcium binding dyes like fluo-4. The increase or decrease in cytotoxicity can give us an insight into what pain modulators can promote or disturb tumour progression in cancer treatment."
"I am a student in Medical Year 1, and I have been working with Dr. Busselberg for over a year. In this lab, we (the students) get to choose our own research projects, write proposals for grants, and design our own experiments, all with help and guidance from Dr. Busselberg. Consequently, while exploring the on-going research and designing our research plans, we learn not only the science, but also independent thinking, planning, and specially analysing the raw data. Currently, my group and I are working on how painkillers such as Aspirin affect the efficiency of anti-cancer drugs, such as Cisplatin."
Dr. Hisham Abdelmotilib
"I joined Dr. Busselberg`s lab in July 2012 as a volunteer researcher. My research interest was studying the effect of anticancer drugs on the calcium current across the cell membranes of neuroblastoma and breast cancer cells. I used the Patch clamp technique to determine the calcium current across the cell membrane. Currently, I am a PhD student in University of Alabama at Birmingham (UAB), USA. I have started my PhD program in August 2013."
"I joined Dr. Büsselberg`s lab through the Biomedical Research Training Program for Nationals as a recent graduate in pharmacology. The main focus of our lab was on intracellular calcium imaging in breast cancer cells. How does calcium signaling in these cells deviate from normal? And what would be the effect of some classic anti-neoplastic drugs on this delicate signaling system? The mixture of techniques used and the methodology of tackling scientific questions gave me a great insight into the field of biomedical research."
"During my time in the Büsselberg lab, I worked on elucidating the role of calcium pathways in the anti-cancer actions of cisplatin. Part of the project aimed to utilize that information in order to determine specific hormonal conditions under which the cells would be most responsive to cisplatin. Being in the lab has allowed me to experience research from multiple perspectives. I gained experience in writing proposals, mastered a variety of lab techniques, and practiced presenting both posters and oral presentations."
"My first experience with research was in Dr. Büsselberg's lab in WCM-Q. I was working with two of my colleagues on a project sponsored by UREP. I learned a lot of basic techniques like cell culture, fluorescence imaging, and patch clamp. In addition, I was able to present my findings in two conferences and I attended a conference in Germany where we presented our results. After successfully finishing the UREP project, we wrote a review article that was published in the Journal of Local and Global Health Science."
"My experience in the lab introduced me to the field of biomedical research. In fact it inspired me to pursue a career in research and discovery. Perhaps the most important experience I gained was systematically reviewing the literature and formulating a key question. This would be followed by designing the study to test hypotheses. Initially the volume of information and deciphering the "language of research" in a given research area seemed challenging but after a while the excitement took over and I could see through the literature to ask the right questions in order to "connect the dots". In the lab, I learnt a whole plethora of techniques ranging from cell culture to immunostaining. I was introduced to both molecular biology and electrophysiology techniques. I learnt to appreciate the importance of patience and persistence and believing in your idea when the going gets hard. Its perfectly normal to be frustrated by your results as many a time they don't make sense and sometimes go completely against your idea. Such experiences taught me the "real" scientific method the hard way. Having an open mind while being objective, humble and curious, I believe, are at the very heart of the scientific method. I also believe such skills can only be learnt when one gets his/her hands dirty in a research lab."
"They say a little knowledge is a dangerous thing, but I believe in putting together little pieces of information to see the bigger picture. This is why I am interested in research – so that I can combine fragments of concepts to understand the functioning of organisms, at a cellular and molecular level and be able to appreciate how things work and why things go wrong when they do."
"Tiny molecules in the human body work together to bring about the huge range of capabilities of man. Figuring out how the interactions between these minuscule entities create the complex functional properties of cells and organs is my field of interest."
"I am currently a first year medical student. With the growing advancement in the medical field, research has become an inseparable part of our education and future practice. I am looking forward to work in this lab to enhance my comprehension of the basic changes occurring during metal toxicity and relating them to the underlying processes of neuronal transmission. Taking part in such a study will further enrich my knowledge aside from exposing me to the methodology of collecting, assessing and interpreting information. PS: I love playing chess :) "
1. Editor-in-Chief and Managing Editor
- "Journal of Local and Global Health Science"; http://www.qscience.com/loi/jlghs/
- Florea AM, Büsselberg D, David Carpenter. Special Issue: “Metals and Disease”. Journal of Toxicology. Volume 2012, Article ID 825354, 2 pages. Hindawi Publishing Corporation doi:10.1155/2012/825354.
- Carpenter DO, Büsselberg D. Editor’s Page: Introduction. Journal of Local and Global Health Science. Vol. 2013,5, Qscience. http://www.qscience.com/doi/pdf/10.5339/jlghs.2013.5
3. Peer-reviewed Articles
- Shevtsova NA, Büsselberg D, Molkov VL, Bischoff AM, Smith JC, Richter DW and Rybak IA . Effects of glycinergic inhibition failure on respiratory rhythm and pattern generation. Progress in Brain Research (under review) 2013.
- Florea AM and Büsselberg D. The two opposite facets of arsenic: Toxin and anticancer drug. Journal of Local and Global Health Science. 2013. http://dx.doi.org/10.5339/jlghs.2013.1
- Florea AM and Büsselberg D. Breast cancer and possible mechanisms of therapy resistance. Journal of Local and Global Health Science. 2013. http://dx.doi.org/10.5339/jlghs.2013.2
- Juntermanns B, Göpelt K, Büsselberg D. Modulation of the Respiratory Rhythm by Gap-Junction Blockers 18-a-Glycyrrhetinic Acid and 18-b-Glycyrrhetinic Acid. Indian Journal of Physiology and Pharmacology. 07/2013; 57(3): 214.
- Florea AM, Taban J, Varghese E, Alost BT, Moreno S, Büsselberg D. Lead (Pb2+) Neurotoxicity: Ion-Mimicry with Calcium (Ca2+) Impairs Synaptic Transmission. A Review with Animated Illustrations of Pre- and Post-synaptic Lead Effects. Journal of Local and Global Health Science. 2013; ttp://dx.doi.org/10.5339/jlghs.2013.4
- Shanmugam V, Wafi A, Al-Taweel N and Büsselberg D. Disruption of Circadian Rhythm Increases the Risk of Cancer, Metabolic Syndrome and Cardiovascular Disease A Review. Journal of Local and Global Health Science. 2013 http://dx.doi.org/10.5339/jlghs.2013.3
- Hagenacker T, Schäfer N, Büsselberg D, Schäfers M. Analgesic ineffectiveness of lacosamide after spinal nerve ligation and its sodium channel activity in injured neurons. European Journal of Pain Article first published online: 11 DEC 2012 DOI: 10.1002/j.1532-2149.2012.00260.x.
- Sadiq S, Ghazala Z, Chowdhury A, Büsselberg D. Metal toxicity at the synapse: presynaptic, postsynaptic and long-term effects. Journal of Toxicology, Special Issue "Metals and Disease" 2012:132671.Epub 2012 Jan 12.
- Florea AM and Büsselberg D. Cisplatin as an anti-tumor drug: cellular mechanisms of activity, drug resistance and induced side effects. Cancers 2011, 3(1), 1351-1371. http://www.mdpi.com/2072-6694/3/1/1351.
- Hagenacker T, Ledwig D, Büsselberg D. Additive inhibitory effects of calcitonin and capsaicin on voltage activated calcium channel currents in nociceptive neurons of rat. Brain Research Bulletin. 2011 Apr 25;85(1-2):75-80. Epub 2011 Feb 16.
- Florea AM, Büsselberg D. Metals and breast cancer: risk factors or healing agents? Journal of Toxicology. 2011(2011)159619. Epub 2011 Jul 24.
- Hagenacker T, Ledwig D, Büsselberg D. Additive inhibitory effects of calcitonin and capsaicin on voltage activated calcium channel currents in nociceptive neurons of rat. Brain Research Bulletin. 2011 Apr 25;85(1-2):75-80.
- Hagenacker T, Hillebrand I, Büsselberg D, Schäfers M. Myricetin reduces voltage activated potassium channel currents in DRG neurons by a p38 dependent mechanism. Brain Research Bulletin. 2010 Oct 30;83(5):292-6.
- Hagenacker T, Hillebrand I, Wissmann A, Büsselberg D, Schäfers M. Anti-allodynic effect of the flavonoid myricetrin in a rat model of neuropathic pain: involvement of p38 and protein kinase C mediated modulation of Ca2+ channels.
- Hagenacker T, Czschik JC, Schäfers M, Büsselberg D. Sensitization of voltage activated calcium channel currents for capsaicin in nociceptive neurons by tumor necrosis factor alpha. Brain Research Bulletin, 2010, 81(1): 157-163.
- Greffrath W, Schwarz St T, Büsselberg D, Treede R-D. Heat-evoked action potential discharges in nociceptive primary sensory neurons of rats. J. Neurophysiology, 2009, 102(1):424-436.
- Florea A-M, Büsselberg D. Anti-Cancer drugs interfere with calcium signaling. Neurotoxicology 2009, 30(5): 803-810.
- Günes DA, Florea A-M Splettstoesser F, Büsselberg D. Co-application of arsenic trioxide (As2O3) and cisplatin (CDDP) on human SY-5Y neuroblastoma cells has differential effects on the intracellular calcium concentration ([Ca2+]i) and cytotoxicity. Neurotoxicology 2009, 30(2): 194-202.
- Florea A-M, Büsselberg, D. Metallic compounds (arsenic trioxide and trimethyltin chloride) interact with calcium homeostasis and trigger cell death. Materialwissenschaft und Werkstofftechnik 2009, 40: 13-16.
- Tomaszewski A, Büsselberg D. SnCl2 reduces voltage gated calcium channel currents of dorsal root ganglion neurones of rats. Neurotoxicology 2008, 29: 958-963.
- Florea A-M, Büsselberg D. Arsenic trioxide in environmentally and clinically relevant concentrations alters calcium homeostasis and induces cell type specific apoptosis in tumour and non-tumour cells. Toxicology letters 2008, 179: 34-42.
- Czeschik JC, Hagenacker T, Schäfers M, Büsselberg D. TNF-a differentially modulates ion channels of nociceptive neurons. Neuroscience Letters 2008, 434: 293-298.
- Hagenacker T, Ledwig D, Büsselberg D. Intracellular calcium ([Ca2+]i) influences pain sensation in peripheral nociceptive systems. Cell Calcium 2008, 43: 215-227.
- Hagenacker T, Büsselberg D. Modulation of intracellular calcium [Ca2+]i influences capsaicin induced currents of TRPV-1 and voltage activated channel currents in nociceptive neurones. J. Pheripheral Nervous System 2007, 12:277-284.
- Splettstoesser F, Florea A-M, Büsselberg D. IP3-receptor-antagonist 2-APB attenuates cisplatin induced Ca2+-influx in HeLa-S3 cells and prevents activation of calpain. Brit. J Pharmacology 2007, 151: 1176-1186.
- Florea A-M, Splettstoesser F, Büsselberg D. Mechanisms of arsenic trioxide (As2O3) induced calcium signals and cellular death in two human SY-5Y Neuroblastoma and 293 embryonic kidney (HEK) cells. TAAP 2007, 220: 292-301.
- Tomaszewski A, Büsselberg D. Cisplatin modulates voltage gated channels in dorsal root ganglion neurones. Neurotoxicology 2007, 28: 49-58.
- Florea A-M, Büsselberg D. Metals and metal compounds: occurrence, use, benefits and toxic cellular effects. Biometals 2006, 19: 419-427.
- Florea A-M, Splettstoesser, F, Dopp, E, Rettenmeier AW, Büsselberg D. Modulation of calcium homeostasis by trimethyltin chloride in human tumor cells: Neuroblastoma SY5Y and cervix adenocarcinoma Hela S3. Toxicology. 2005, 216: 1-8.
- Hagenacker T, Splettstoesser F, Greffrath W, Treede, R-D, Büsselberg D. Capsaicin blocks voltage-gated calcium channel currents of small DRG neurones. Brain Research 2005, 1062: 74-85.
- Florea A-M, Büsselberg D. Toxic effects of metals: modulation of intracellular calcium homeostasis. Materialwissenschaft und Werkstofftechnik 2005, 36: 757-760.
- Florea A-M, Dopp E, Büsselberg D. Elevated calcium transients by trimehtyltin chloride in HeLa cells: types and levels of elevation. Cell Calcium 2005, 37: 251-258.
- Splettstoesser F, Bonnet U, Wiemann M, Bingmann, D, Büsselberg D. Modulation of voltage gated channel currents by Harman and Harmaline Brit. J Phar-macoL 2005, 144: 52-58.
- Büsselberg D, Bischoff AM, Richter D W. Failure of glycine inhibition changes bursting behaviour of respiratory neurones. Neuroscience 2003, 122/3: 831-841.
- Mohanakumar KP, Samanta Ray S, Büsselberg D. The parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydro-pyridine on membrane currents and intra-synaptosomal calcium. Neuroscience Research Communications 2002, 30/1: 35-42.
- Dutschmann M, Bischoff AM, Richter DW, Büsselberg D. Histaminergic Modulation of the respiratory network via H1, H2 and H3 receptors analysed in an in situ mouse. Pflügers Archiv, European J. Physiology 2003, 445: 570-576.
- Büsselberg D, Bischoff, AM, Becker, K, Becker, C.-M, Richter DW. Respiratory rhythm in mutant oscillator mice (Glra 1spd-ot / Glra 1spd-ot). Neuroscience letters 2001, 316/2: 99-102.
- Büsselberg D, Bischoff AM, Paton, JFR, Richter DW. Reorganisation of the respiratory network after loss of glycinergic inhibition. Pflügers Archiv, Eupean J. Physiology 2001,441: 444-449.
- Schwarz S, Greffrath W, Büsselberg D, Treede R-D. Inactivation and tachy-phylaxis of heat-evoked inward currents in nociceptive primary sensory neurones in rats. J Physiology (Lond) 2000, 528 (3): 539-549.
- Richter DW, Mironov SI, Büsselberg D, Lalley PM, Bischoff AM, Wilken B. Respiratory rhythm generation: plasticity of a neuronal network Neuroscientist 2000, 6(3): 181-198.
- Wiemann M, Schirrmacher K, Büsselberg D. Interference of lead with the calcium release activated calcium flux of osteoblast-like cells. Calcified Tissue Int. 1999, 65: 479-485.
- Kirschstein T, Greffrath W, Büsselberg D, Treede R-D. Inhibition of rapid heat responses in nociceptive primary sensory neurones of the rat by vanilloid receptor antagonists. J Neurophysiol 1999, 82: 2853-2860.
- Schirrmacher K, Büsselberg D, Langosch JM, Bingmann D. Effects of (±)-kavain on voltage-activated inward currents of dorsal root ganglion cells from neonatal rats. European J. Neuropsycopharmacol 1999, 9: 171-176.
- Wiemann M, Büsselberg D, Schirrmacher K, Bingmann D. A calcium release activated calcium influx in primary cultures of rat osteoblast-like cells. Calcified Tissue Int. 1998, 63: 154-159.
- Büsselberg D, Schirrmacher K, Domann R, Wiemann M. Lead interferes with calcium entry through Membrane pores. Fresenius´ J. Analyt. Chem 1998, 361: 372-376.
- Schirrmacher K, Wiemann M, Bingmann D, Büsselberg D. Effects of lead, mercury and methyl-mercury on gap junctions and [Ca2+]i in bone cells. Calcified Tissue Int. 1998, 63:134-139.
- Diewald L, Heimrich B, Büsselberg D, Watanabe T, Haas HL. Histaminergic system in co-cultures of hippocampus and posterior hypothalamus: a morphological and electrophysiological study in the rat. European J Neuroscience 1997, 9: 2406-2413.
- Gawrisch E, Leonhardt R, Büsselberg D. Voltage-activated calcium channel currents of rat dorsal root ganglion cells are reduced by trimethyl lead Toxicology letters 1997, 92: 117-122.
- Kirschstein T, Büsselberg D, Treede RD. Coexpression of heat-evoked inward currents in acutely dissociated rat dorsal root ganglion neurons. Neuroscience letters 1997, 231: 1-4.
- Domann R, Wunder L, Büsselberg D. Lead reduces calcium entry after depo-larisation without passing the cell membrane in cultured DRG neurons: FURA-2 measurements. Cell. Molec. Neurobiol. 1997, 17/3: 305-314.
- Leonhardt R, Pekel M, Platt B, Haas HL, Büsselberg D. Mercury compounds reduce voltage activated ion channel currents. Metal Ions in Biology and Medicine 1996, 4: 204-206.
- Büsselberg D. Actions of lead on neuronal membrane currents and synaptic plasticity. Metal Ions in Biology and Medicine 1996, 4: 241-243.
- Büsselberg D. Calcium channels as target sites of heavy metals. Toxicology letters 1995, 82/83: 255-262.
- Leonhardt R, Haas HL, Büsselberg D. Voltage gated calcium, potassium and sodium channel currents of rat DRG neurons are reduced by methylmercury. Naunyn Schmiedebergs Archievs of Pharmacology 1996, 354/4: 532-538.
- Leonhardt R, Pekel M, Platt B, Haas HL, Büsselberg D. Voltage-activated calcium channel currents of rat DRG neurons are reduced by mercuric chloride (HgCl2) and methylmercury (CH3HgCl). Neurotoxicology 1996, 17/1: 85-91.
- Platt, B, Carpenter, DO, Büsselberg, D, Reymann, KG. and G. Riedel Aluminum impairs hippocampal long-term potentiation in rats in vitro and in vivo Exp. Neurol. 1995, 134: 1-14. IF: 4.156
- Platt B, Haas H, Büsselberg D. Aluminium reduces glutamate-activated currents of rat hippocampal neurons. NeuroReport, 1994, 5: 2329-2332.
- Büsselberg D, Pekel M, Michael D, Platt B. Mercury (Hg2+) and zinc (Zn2+): Two divalent cations with different actions at voltage activated calcium channel currents. Cell. Molec. Neurobiol. 1994, 14: 675-687.
- Büsselberg D, Michael D, Platt B. Pb2+ reduces voltage- and NMDA-activated calcium channel currents. Cell Molec. Neurobiol 1994, 14: 711-722.
- Platt B, Büsselberg D. Actions of aluminum on voltage activated calcium channel currents. Cell Molec. Neurobiol 1994, 14: 819-829.
- Platt B, Büsselberg D. Combined actions of Pb2+, Zn2+ and Al2+ on voltage activated calcium channel currents. Cel.l Molec. Neurobiol. 1994, 14: 831-840.
- Platt B, Haas H, Büsselberg D. Extracellular pH modulates aluminium-blockade of mammalian voltage-activated calcium channel currents. NeuroReport 1993, 4: 1251-1254.
- Pekel M, Platt B, Büsselberg D. Effects of mercury (Hg2+) on voltage activated calcium channel currents in Aplysia and cultured rat neurons. Brain Res. 1993, 632: 121-126.
- Büsselberg D, Platt B, Michael D, Carpenter DO, Haas HL. Mammalian voltage activated calcium channel currents are blocked by Pb2+, Zn2+ and Al2+. J Neurophysiology 1994, 71: 1491-1497.
- Büsselberg D, Platt B, Haas HL, Carpenter DO. Voltage-gated calcium channel currents of rat dorsal root ganglion (DRG) cells are blocked by Al2+. Brain Res. 1993, 622:163-168.
- Uteshev V, Büsselberg D, Haas HL. Pb2+ modulates the NMDA-receptor-channel complex. Naunyn Schmiedebergs Archiv of Pharmacology, 1993, 347:209-213.
- Hori N, Büsselberg D, Matthews R, Parson P, Carpenter DO. Lead blocks LTP by an action not at NMDA receptors. J Experimental Neurology, 1993, 119:192-197.
- Büsselberg D, Evans M L, Haas H L, Carpenter DO. Blockade of mammalian and invertebrate calcium channels by lead Neurotoxicology, 1993, 14:249-258.
- Büsselberg D, Michael D, Evans M L, Carpenter DO, Haas HL. Zinc (Zn2+) blocks voltage gated calcium channels in cultured rat dorsal root ganglion cells. Brain Res., 1992, 593:77-81.
- Evans M L, Büsselberg D, Carpenter D O. Lead blocks the calcium current of cultured dorsal root ganglion cells. Neuroscience letters, 1991, 129:103-106.
- Schönrock B, Büsselberg D, Haas H L. Properties of tuberomammillary hista-mine neurones and their response to galanin. Agents and Actions, 1991, 33, 135-137.
- Büsselberg D, Evans M L, Rahmann H, Carpenter DO. Lead and Zinc block a voltage activated calcium channel of Aplysia neurons. J Neurophysiology, 1991, 65(4): 786-795.
- Büsselberg D, Evans M L, Rahmann H, Carpenter DO. Effects of inorganic and triethyllead and inorganic mercury on the voltage activated calcium channel of Aplysia neurons. Neurotoxicology, 1991, 12:733-744.
- Büsselberg D, Evans ML, Rahmann H, Carpenter DO. Zn2+ blocks the voltage activated calcium current of Aplysia neurons and changes its kinetics. Neuroscience letters, 1990, 117-122.
- Büsselberg D, Evans ML, Rahmann H, Carpenter DO. Lead inhibits the voltage activated calcium current of Aplysia neurons. Toxicology letters, 1990, 51:51-57.
- Büsselberg D, Carpenter DO, Sugita M, Araki S, Satake M, Rahmann H. Effects of exogenous lipid on excitability of Aplysia neurons. Biomedical Research, 1990, 11 (2):77-86.
- Büsselberg D, Evans ML, Rahmann H, Carpenter DO. Effects of exogenous gangliosides and cholesterol application of Aplysia neurons. Memb Biochem, 1989, 8:19-26.
4. Books, Book Chapters and reviews
- Chapter 9. Calcium ([Ca2+]i) signaling as a key-target for cancer treatment. Florea AM, Büsselberg D. Book: Calcium Signaling ISBN: 978-1-61324-313-8 Editor: Masayoshi Yamaguchi, ©2011 Nova Science Publishers, Inc.
- Mock E P, Juntermanns B, Göpelt K, Büsselberg D. Various „drugs“ influence the respiratory rhythm. In: Neuro-Visionen 4; K.-A. Hossmann (Ed.), Verlag Ferdinand Schöningh, Paderborn, München, Wien, Zürich, 2007, 39-40.
- Hagenacker T, Ledwig D, Neuendorf A, Czeschik C, Schäfers M, Büsselberg D. New pharmaceutic interventions in the therapy of chronic pain. In: Neuro-Visionen 4; K.-A. Hossmann (Ed.), Verlag Ferdinand Schöningh, Paderborn, München, Wien, Zürich, 2007, 133-135.
- Günes DA, Florea A-M, Splettstoesser F, Kunz I, Büsselberg D. Anti-cancer drugs elevate intracellular calcium by different mechanisms. In: Neuro-Visionen 4; K.-A. Hossmann (Ed.), Verlag Ferdinand Schöningh, Paderborn, München, Wien, Zürich, 2007, 35-36.
- Neuendorf A, Hagenacker T, Büsselberg D. Differential modulation of voltage gated channels by capsaicin. In: Neuro-Visionen 3; K.-A. Hossmann (Ed.), Verlag Ferdinand Schöningh, Paderborn, München, Wien, Zürich, 2006, 99-100.
- Florea A-M, Splettstoesser F, Tomaszewski A, Büsselberg D. Anti cancer drugs (As2O3 and cis-platin) interfere with calcium signals in in tumour cells. In: Neuro-Visionen 3; K.-A. Hossmann (Ed.), Verlag Ferdinand Schöningh, Paderborn, München, Wien, Zürich, 2006, 34-36.
- Mock E P, Göpelt K, Büsselberg D. Calcium channel modulators influence breathing. In: Neuro-Visionen 3; K.-A. Hossmann (Ed.), Verlag Ferdinand Schöningh, Paderborn, München, Wien, Zürich, 2006, 32-33.
- Florea A-M, Splettstoesser F, Dopp E, Rettenmeier A W, Büsselberg D. The environmentally relevant trimethyltin (TMT) influences calcium homeostasis in ex-citable and non-excitable cell systems. In: Neuro-Visionen 2; K.-A. Hossmann (Ed.), Verlag Ferdinand Schöningh, Paderborn, München, Wien, Zürich, 2005, 23-25.
- Juntermanns B, Göpelt K, Splettstoesser F, Büsselberg D. The respiratory rhythm changes under blockade of gap junctions. In: Neuro-Visionen 2; K.-A. Hossmann (Ed.), Verlag Ferdinand Schöningh, Paderborn, München, Wien, Zürich, 2005, 51-53.
- Hagenacker T, Splettstoesser F, Büsselberg D. Pain modulation by voltage gated calcium channels. In: Neuro-Visionen 2; K.-A. Hossmann (Ed.), Verlag Ferdinand Schöningh, Paderborn, München, Wien, Zürich, 2005, 145-146.
- Büsselberg D. Actions of Metals on Membrane Channels, Calcium Homeostasis and Synaptic Plastivity. In: Organometal and Metalloid Specism in the Environment: Analysis, Distribution, Processes and Toxicological Evaluation; A. V. Hirner & H. Emons (Eds.), Springer, Wien, New York, 2004, 259-281.
- Büsselberg D. Blei reduziert Gedächtnisleistungen: historische und aktuelle Aspekte; Stand der Forschung. In: Kulturring, Jahresberichte, 1996, 73:18-20.
- Haas H L, Büsselberg D. Neurotoxikologie in vitro mit Hilfe der Elektrophysiologie. In: Ersatz- und Ergänzungsmethoden zu Tierversuchen; Schöffl, H., Spielmann, H. und Tritthart, H.A., Springer, Wien, New York, 1996, 65-69.
- Büsselberg D. Bleiwirkungen auf neuronale Membranströme und synaptische Plastizität. Habilitationsschrift; Verlag Dr. Köster, Berlin; ISBN 3-89574-064-0; 1995.
- Haas H L, Büsselberg D. Recording Chambers - Slices. In: Practical electro-physiological methods. A guide for in vitro studies in vertebrate neurobiology; Kettenmann, H. and Grantyn R. (Eds.), Wiley-Liss, New York, 1992, p. 16-19.
Chemotherapy and Intracellular Calcium
- Dr. Dr. Ana Maria Florea, Heinrich-Heine- University Düsseldorf, Germany
- Prof. Dr. Guido Reifenberger Heinrich-Heine- University, Düsseldorf, Germany
- Dr. Osvaldo Padilla, Texas Tech Paul L. Foster School of Medicine, Texas, USA.
- Prof. Dr. Volker Ehemann, DKFZ, Heidelberg, Germany
- Dr. Thompson Sarkodi-Gyan. University of Texas at El Paso, Texas, USA
Cellular Mechanisms of Pain Modulation
- Dr. Tim Hagenacker, University of Essen, Germany
- Dr. Maria Schäfers, University of Essen, Germany
- Dr. Wolfgang Greffrath, University of Manheim, Germany
- Prof. Dr. Rolf-Detlev Treede, University of Manheim, Germany
Ca2+-signals after the application of an anti-cancer drug
Localization of IP3-receptors at the cellular membrane