The Histology core provides services to the research community, primarily to WCM-Q researchers. The core services also support the collaborative research with other biomedical institutes in Qatar. The core has well trained staff to carry out the different services provided.
- Sample preparation, and embedding of tissues for both paraffin and Cryoembedding.
- Sectioning of samples (both paraffin sectioning and cryosectioning).
- Staining with various histological stains (e.g. Hematoxylin and Eosin, Masson Trichrome, Giemsa staining of chromosome, etc.)
- Immunohistochemical staining using autostainer (Dako). We also can provide regular immunohistochemistry. Staining can be performed for single antigen or co-staining for different antigens.
- Luminex 200 for measurement of biomarkers.
- Leica Automated tissue processor (Leica ASP 200S)
- Leica Tissue Embedder (Leica EG1150H, EG1150 C)
- Leica Motorized Microtome (Leica RM2255)
- Leica Cryostat (Leica CM 3050S)
- Dako Autostainer Link-48
The Dako autostainer performs automated immunohistochemistry with Dako reagents and custom antibodies. It also contains a hybridizer to carry out special stains such as FISH and CISH.
- Ziess Axioskope with MRC5 CCD digital Camera and image capture software
- Palm Micro Beam Zeiss with Axio Observer with Axia cam MRm Microscope
The Palm system is a motorized Laser Capture Microdissection (LCM) system. It can be used to isolate a specific region of the tissue section or cells in culture plate for further analysis such as DNA, RNA or protein isolation.
- Luminex 200 (Life Technologies)
This is a flexible analyzer able to simultaneously measure up to 100 analytes in a single microplate well. It requires small sample size. Bioassay can be performed in different formats such as: nucleic acid assays, receptor ligand assays, immunoassays and enzymatic assays.
Nasrin Mesaeli, PhD
Director of Molecules, Genes and Cells Course
Director of Histology Core
Associate Professor Biochemistry
Tel: +974 4492 8327
Fax: +974 4492 8422
Office Phone: 4492-8950