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Services

Sanger Sequencing

In this service, the core will set up the sequencing reactions, clean the samples and sequence them on the ABI 3130xl Genetic Analyzer.

Genome Sequencing

In this service, the core will provide library construction and sequencing of whole genomes.  We provide re-sequencing services using the Illumina Hiseq 4000 or the Illumina Miseq as well as de novo sequencing using the Illumina HiSeq 4000, or/and PacBio RS II, or/and 10x Chromium.

Transcriptome Sequencing

In this service, the core will provide library construction and sequencing of:
  • total RNA or mRNA using the Illumina HiSeq 4000
  • small RNA using the Illumina MiSeq 
  • full-length transcripts (Iso-Seq) using the PacBio RSII

Additionally, the core performs rRNA depletion for total RNA and Globin depletion for blood samples before library construction. 

Targeted Sequencing

This service includes library construction and sequencing of whole exome and targeted gene panels on the Illumina NGS platforms. 
 

Genotyping

The genotyping service includes array genotyping using the Illumina iScan System and Genotyping-By-Sequencing (GBS) on the Illumina Hiseq 4000.  
 

Metagenomic Profiling

For microbial profiling, the core provides 16s ribosomal RNA amplification, library construction and sequencing on the Illumina MiSeq.  The core also provides whole genome sequencing on the Illumina Hiseq 400 of genomic DNA recovered from environmental samples.
  
To request a Services please contact:

Yasmin Mohamoud, M.Sc., 
Manager, Genomics Core 
Phone: (+974) 4492 8474
E-mail: yam2012@qatar-med.cornell.edu